Development of a new recombinant antibody, selected by phage-display technology from a celiac patient library, for detection of gluten in foods

Development of a new recombinant antibody, selected by phage-display technology from a celiac patient library, for detection of gluten in foods

Blog Article

Gluten, a group of ethanol-soluble proteins present in the endosperm of cereals, is extensively used in the food industry due to its ability to improve dough properties.However, gluten is also associated with a range of gluten-related diseases (GRDs), such as wheat allergies, celiac disease, and gluten intolerance.The rumchata proof recommended treatment for GRDs patients is a gluten-free diet.

To monitor adherence to this diet, it is necessary to develop gluten-detection systems in food products.Among the available methods, immunodetection systems are the most popular due to their simplicity, reproducibility, and accuracy.The aim of this study was to generate novel high-affinity antibodies against gluten to be used as the primary reactant in an enzyme-linked immunosorbent assay (ELISA) test.

These antibodies were developed by constructing an immune read more library from mRNA obtained from two celiac patients with a high humoral response to gluten-related proteins.The resulting library (composed by 1.1x107) was subjected to selection against gliadin using phage display technology.

Following several rounds of selection, the Fab-C was selected, and demonstrated good functionality in ELISA tests, presenting a limit of detection of 15 mg/kg for detection of gluten in spiked mixtures and food products.The methodology can discriminate gluten-free products according to the current legislation.